Phospho mtor ser2448

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August undefined, 2024

WebThe Phospho-mTOR (Ser2448) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2448) Antibody has been validated for the following applications: Western Blot. Phospho-mTOR (Ser2448) Antibody detects … WebIn conclusion, it is recommended that Ser2448 is an inadequate measure and that preferential analysis of mTORC1 activation should focus on the downstream and effector …

Phosphorylation of mammalian target of rapamycin (mTOR) at

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … Web斯氏毛滴虫pcr检测试剂盒厂家上海联祖生物相关产品:28s rrnp检测试剂盒实验流程1.实验前标准品、试剂及样本的准... flow fe

Human and Mouse Phospho-mTOR (S2448) ELISA RayBiotech

Web上海康朗生物科技有限公司主要致力于“Anti-Phospho-mTOR (Ser2448)抗体”的生产销售。多年的“Anti-Phospho-mTOR (Ser2448)抗体”生产与销售的经验，与各行业新老用户建立了 … WebmTOR抑制剂的筛选方法. 分析：OD值越小，mTOR的Ser2448磷酸化程度越低，抑制剂作用越强。. （2）荧光共振能量转移（FRET）. 荧光共振能量转移是指两个荧光发色基团在足够靠近（7~10nm）时，当供体分子吸收一定频率的光子后被激发到更高的电子能态，在该电子回 … WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. flow fci

Anti-mTOR (phospho S2448) antibody (ab1093) Abcam

MSD Phospho( Ser2448 )/Total mTOR Assay - Meso Scale

WebPhospho-mTOR (Ser2448) (49F9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human … WebJan 1, 2024 · Starting on day 15, mice were treated three times weekly with 1 mg/tumor Mito-MGN administered via an intratumoral injection in a 50 μL volume, and the control mice were injected with a vehicle of 20% ethanol in phosphate buffered saline. green candle meaning stocksWebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor. flowfeed

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Phospho mtor ser2448

phosphorylation-independent mtorc1 inhibition by the ... - 豆丁网

WebPrinciple of Assay. This mTOR (phospho Ser2448) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2448) and the effects that certain stimulation conditions have on mTOR (phospho Ser2448) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2448) concentration is achieved by an indirect … WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) …

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WebPhospho-mTOR (Ser2448) Antibody (46-9718-42) in Flow TOP: Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 Functional Grade … WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with …

WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation … WebThe Phospho-mTor (Ser2448) assay measures mTor when phosphorylated at Ser2448. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-mTor (Ser2448) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor.

WebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody [EPR426 (2)] (ab109268) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … WebApr 5, 2024 · Several upstream signals, including ERK, converge on the phosphorylation and inhibition of Tuberous Sclerosis Complex (TSC) to activate mTORC1 [20, 21]. mTOR functions as the catalytic subunit of two individual complexes, known as …

WebJan 9, 2024 · Both mTORC1 and mTORC2 are regulated by phosphorylation, complex formation and localization. So far, the role of p70S6K-mediated mTOR S2448 phosphorylation has not been investigated in detail. Here, we report that endogenous mTOR phosphorylated at S2448 binds to both, raptor and rictor.

WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for … green candle meaning magicWebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3 … flowfeed bentonvilleWebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, … green candle money magic spellWebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, which indicates the mTORC1... green candle meaning in witchcraftWebProduct Description This peptide is used to specifically block #2971 Phospho-mTOR (Ser2448) Antibody reactivity. Quality Control The quality of the peptide was evaluated by … green candies in candy crushWebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR … green candle meaning spirituallyWebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts … flow feedback with recipe 流量反馈